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the termisolationrefers to the separation of a strain from a natural, mixed population of livingmicrobes, as present in the environment, for example in water orsoil flora, or from living beings withskin flora,oral floraorgut flora, in order toidentifythe microbe(s) of interest. Historically, thelaboratory techniquesof isolation first developed in the field ofbacteriologyandparasitology(during the 19th century), before those invirologyduring the 20th century. Methods of microbial isolation have drastically changed over the past 50 years, from a labor perspective with increasing mechanization, and in regard to the technology involved, and hence speed and accuracy.

Proteinpurificationis a seriesofprocesses intended toisolateone or a few proteins from a complex mixture, usually cells, tissues or whole organisms

Listofpurificationmethods in chemistry

substances. Pure resultsofa successfulpurificationprocess are termedisolate. The following listofchemicalpurificationmethods should not be considered

Isolate

chemicalpurificationprocess Proteinisolate, the resultofproteinpurification, whichisolatesa single typeofprotein from a complex mixtureIsolate(biology)

Microorganisms occur in natural environment like soil. ... A culture which contains just one species of microorganism is called a pure culture. The process of obtaining a pure culture by separating one species of microbe from a mixture of other species, is known as isolation of the organisms