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Basic principle behind chromotography |
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Answer» In this process the heavy particles sattle down and the lighter particles up so thos can separate Principle: The samples are subjected to flow by mobile liquid onto or through the stable stationary phase. The sample components are separated into fractions based on their relative affinity towards the two phases during their travel.The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer. When is solvent?? |
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