DNA being hydrophilic cannot pass through the cell membrane of a host

1.	DNA being hydrophilic cannot pass through the cell membrane of a host cell. Explain how does recombinant DNA get introduced into the host cell to transform the latter.
Answer» rDNA is introduced into the host cell by the following methods: (i) Heat shock method: In this method, DNA is treated with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. The rDNA is forced into the cell by incubating the cell with rDNA on ice, followed by placing them briefly at 42°C (heat shock) and then putting them back on ice. (ii) Micro-injection: In this method, the rDNA is directly injected into the nucleus of an animal cell. (iii) Gene gun/Biolistics: In this method, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.

DNA being hydrophilic cannot pass through the cell membrane of a host cell. Explain how does recombinant DNA get introduced into the host cell to transform the latter.

Answer»

rDNA is introduced into the host cell by the following methods:

(i) Heat shock method: In this method, DNA is treated with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. The rDNA is forced into the cell by incubating the cell with rDNA on ice, followed by placing them briefly at 42°C (heat shock) and then putting them back on ice.

(ii) Micro-injection: In this method, the rDNA is directly injected into the nucleus of an animal cell.

(iii) Gene gun/Biolistics: In this method, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.

Discussion

No Comment Found

Related InterviewSolutions

What is meant by coagulation of a colloidal solution? Describe briefly any three methods by which coagulation of lyophobic sols can be carried out.
What is meant by coagulation of a colloidal solution? Name any method by which coagulation of lyophobic sols can be carried out.
Classify the following contraceptives measures into different methods of birth control.(1) Vasectomy(2) tubectomy(3) Saheli(4) Diaphragms(5) Cervical caps(6) Condoms
An object is placed at a distance of 30 cm from a concave lens of focal length 15 cm. List four characteristics (nature, position etc) of the image formed by the lens.
Explain the following: (i) CH3COOH is a weak acid. (ii) Propene undergoes addition reaction. (iii) The gas stoves have inlets for air.
Accumulation of harmful chemicals in our body can be avoided. Explain how this can be achieved ?
State the changes that take place in the uterus when: (i) Implantation of embryo has occurred (ii) Female gamete/egg is not fertilised
A hollow metal sphere of radius 5cm is charged such that the potential on its surface is 10V. What is the potential at the centre of the sphere?
Ms. Raveena Sen is an IT expert and a freelancer. She recently used her skills to access the Admin password for the network server of Super Dooper Technology Ltd. and provided confidential data of the organization to its CEO, informing him about the vulnerability of their network security. Out of the following options (i) to (iv), which one most appropriately defines Ms. Sen? Justify the reason for your chosen option: (i) Hacker (ii) Cracker (iii) Operator (iv) Network Admin
An elevator descends into a mine shaft at the rate of 6 m/min. If the descent starts from 10 above the ground level, how long will it take to reach -350 m?