Explain about the principle involved in chromatography. Give its types

1.	Explain about the principle involved in chromatography. Give its types.
Answer» 1. The principle behind chromatography is selective distribution of the mixture of organic substances between two phases-a stationary phase and a moving phase. The stationary phase can be a solid or liquid while the moving phase is a liquid or a gas. 2. if the stationary phase is solid, the basis is adsorption and when it is a liquid, the basis is partition. 3. Chromatography is defined as technique for the separation of a mixture brought about by differential movement of the individual compound through porous medium under the influence of moving solvent. 4. The various methods of chromatography are: Column chromatography (CC) Thin layer chromatography (TLC) Paper chromatography (PC) Gas liquid chromatography (GLC) Ion exchange chromatography

Explain about the principle involved in chromatography. Give its types.

Answer»

1. The principle behind chromatography is selective distribution of the mixture of organic substances between two phases-a stationary phase and a moving phase. The stationary phase can be a solid or liquid while the moving phase is a liquid or a gas.

2. if the stationary phase is solid, the basis is adsorption and when it is a liquid, the basis is partition.

3. Chromatography is defined as technique for the separation of a mixture brought about by differential movement of the individual compound through porous medium under the influence of moving solvent.

4. The various methods of chromatography are:

Column chromatography (CC)
Thin layer chromatography (TLC)
Paper chromatography (PC)
Gas liquid chromatography (GLC)
Ion exchange chromatography

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