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Explain Hill’s reaction. |
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Answer» Robert Hill proved that the source of oxygen evolved during photosynthesis is water and not carbon dioxide. Hence, it is called Hill’s Reaction. 1. In this experiment, Hill cultured isolated chloroplasts in a medium containing CO2 free water, haemoglobin and ferric compound. 2. Ferric salts and haemoglobin were added in the medium as hydrogen and oxygen acceptors respectively. 3. When the suspension was illuminated, he observed that haemoglobin turned into oxyhaemoglobin (red colour). 4. This confirmed that water must have oxidized releasing O2, that reacted with haemoglobin. Reduction of ferric compound was also indicated by change in colour. 5. The H2O molecule oxidized to evolve O2 as a byproduct. Thus, Hill proved that the source of evolving O2 is H2O and not CO2 . 6. This process of splitting up of water molecules under the influence of light in the presence of chlorophyll is called Photolysis of water or Hill Reaction. |
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