1.

Explain sequentially the process of 'Translation' in a prokaryote. Name the cellular factory where this process occurs.

Answer»

(1) Small subunit of ribosome encounters mRNA and protein synthesis process begins. 2 sites in the large subunit for subsequent amino acids to bind to and thus be close enough to each other for the formation of the peptide bond. 

(2) The order and sequence of the amino acid are defined by the bases in m-RNA. 

(3) In the first phase the amino acid are activated in the presence of ATP and linked to t-RNA- (aminoacylation of t-RNA) 

(4) Ribozyme/Ribosome acts as a catalyst for formation of peptide bond. 

(5) A translational unit has a sequence of RNA that is flanked by start codon (AUG) at 5' end and stop codon at 3'end (UAA, UAG, UGA). 

(6) Initiation-ribosome binds to m-RNA at start codon (recognized by tRNA). 

(7) Elongation phase: Amino acids linked to tRNA bind to appropriate codon of mRNA by complementary base pairing. 

(8) Ribosome moves codon to codon along mRNA- polypeptide sequence formed as dictated by DNA & represented by mRNA. 

(9) At the end termination occurs by a release factor which binds to stop codon releasing the polypeptide. 

Ribosome is the cellular factory.



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