Explain the role of DNA dependent RNA polymerase in initiation, elonga

1.	Explain the role of DNA dependent RNA polymerase in initiation, elongation and termination during transcription in bacterial cell.
Answer» The DNA dependent RNA polymerase helps in DNA replication by catalysing the polymerisation in only one direction, i.e., 5′→3′. In bacteria, the RNA polymerase has co-factors b, b′, a, a′, w and s which catalyse the process. Initiation: s (sigma) factor recognises the start signal and promotor region on DNA and the s (sigma) with RNA polymerase binds to the promoter and initiate transcription. Elongation: The RNA polymerase after initiation of RNA transcription loses the s-factor but continues the process of RNA formation. Termination: Once the RNA polymerase reaches the termination region of DNA, the RNA polymerase is separated from DNA–RNA hybrid, as a result nascent RNA separates. This process is called termination which is facilitated by certain termination factor ρ.

Explain the role of DNA dependent RNA polymerase in initiation, elongation and termination during transcription in bacterial cell.

Answer»

The DNA dependent RNA polymerase helps in DNA replication by catalysing the polymerisation in only one direction, i.e., 5′→3′. In bacteria, the RNA polymerase has co-factors b, b′, a, a′, w and s which catalyse the process.

Initiation: s (sigma) factor recognises the start signal and promotor region on DNA and the s (sigma) with RNA polymerase binds to the promoter and initiate transcription.

Elongation: The RNA polymerase after initiation of RNA transcription loses the s-factor but continues the process of RNA formation.

Termination: Once the RNA polymerase reaches the termination region of DNA, the RNA polymerase is separated from DNA–RNA hybrid, as a result nascent RNA separates. This process is called termination which is facilitated by certain termination factor ρ.

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