How are recombinant vectors created? Why is only one type of restricti

1.	How are recombinant vectors created? Why is only one type of restriction endonuclease required for creating one recombinant vector?
Answer» The construction of recombinant DNA is done by linking a gene encoding antibiotic resistance with a native plasmid. These plasmid DNA act as vectors to transfer the piece of DNA attached to it. Only one type of restriction endonuclease is required for creating recombinant vector because when cut by the same enzyme, the resultant DNA fragments have the same sticky ends, which can be joined together using DNA ligases.

How are recombinant vectors created? Why is only one type of restriction endonuclease required for creating one recombinant vector?

Answer»

The construction of recombinant DNA is done by linking a gene encoding antibiotic resistance with a native plasmid. These plasmid DNA act as vectors to transfer the piece of DNA attached to it.

Only one type of restriction endonuclease is required for creating recombinant vector because when cut by the same enzyme, the resultant DNA fragments have the same sticky ends, which can be joined together using DNA ligases.

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How are recombinant vectors created? Why is only one type of restriction endonuclease required for creating one recombinant vector?

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