Question : A_(3)-_(5)B C_(5)-_(3)D AB and CD represent two strands of a DNA molecule. When this molecule undergoes replication, a replication fork is formed between A and C in the above. i. Name the template strands for replication. ii. Using which strand as the template, will there be continuous synthesis of a complementary DNA strand? iii. Complementary to which strand will Okazaki segments get synthesised. iv. What are template strands and Okazaki pieces? v. In which direction is a new strand synthesised?

Question : A_(3)-_(5)B C_(5)-_(3)D AB and CD represent two strands o

1.	Question : A_(3)-_(5)B C_(5)-_(3)D AB and CD represent two strands of a DNA molecule. When this molecule undergoes replication, a replication fork is formed between A and C in the above. i. Name the template strands for replication. ii. Using which strand as the template, will there be continuous synthesis of a complementary DNA strand? iii. Complementary to which strand will Okazaki segments get synthesised. iv. What are template strands and Okazaki pieces? v. In which direction is a new strand synthesised?
Answer» Solution :i. COMPLEMENTARY strands. ii. ONE of the template strands with 3.`rarr`5. polarity. The synthesis of DNA is continuous. iii. Other template strand with polarity 5.`rarr`3., the synthesis of DNA is discontinuous. i.e., short stretches of DNA are SYNTHESISED. iv. Template - It is for the synthesis of a new complementary strand. Okazaki pieces: It is discontinuous FRAGMENTS of DNA. It is Y shaped fragment. v. The new strand is synthesised in 5.`rarr`3. direction.