Question : Describe the procedure involved in Blue-White colony select

1.	Question : Describe the procedure involved in Blue-White colony selection methods.
Answer» Solution :Blue-White Colony Selection Method is a powerful method used for screening of RECOMBINANT plasmid. In this method, a reporter GENE lacZ is INSERTED in the vector. The lacZ encodes the ENZYME `beta`-galactosidase and contains several recognition SITES for restriction enzyme. `beta`-galactosidase breaks a synthetic substrates called X-gal (5-bromo-4-chloroindolyl- `beta`-Dgalacto-pyranoside) into an insoluble blue coloured product. If a foreign gene is inserted into lacZ, this gene will be inactivated. Therefore, no-blue colour will develop (white) because `beta`-galactosidase is not synthesized due to inactivation of lacZ. Therefore, the host cell containing r-DNA form white coloured colonies on the medium contain X-gal, whereas the other cells containing non-recombinant DNA will develop the blue coloured colonies. On the basis of colony colour, the recombinants can be selected.

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