Students while performing the experiment were given two different vectors in two different bacterial colonies being cultured in a chromogenic substrate. They observed that the bacterial colonies with cloning vector A were colourless while those with coloning vector B were blue in colour. Explain the phenomenon and the procedure behind the fact that the students were trying to figure out.

Students while performing the experiment were given two different vect

1.	Students while performing the experiment were given two different vectors in two different bacterial colonies being cultured in a chromogenic substrate. They observed that the bacterial colonies with cloning vector A were colourless while those with coloning vector B were blue in colour. Explain the phenomenon and the procedure behind the fact that the students were trying to figure out.
Answer» Students were working to differentiate recombinants and non-recombinants DNA on the basis of production of colour in the presence of a chromogenic substrate. During this phenomenon, a recombinant DNA is inserted within the coding sequence of an enzyme 1-galactosidase that results in an inaction of the enzyme. Due to which, the bacterial colonies having plasmid inserted within it shows no colouration at all and those without plasmid tends to show blue colour.

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