InterviewSolution
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What Are Different Sterilization Methods Used In Laboratory? |
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Answer» The most common methods of sterilization PRACTISED in lab are: Dry heat: Specimen containing bacteria is exposed to high temperature Wet heat: Pressurised steam is used to kill MICROBES, for example, autoclave that is like pressure cooker that produces steam. Filtration: Filtration is used where filters are as small as 0.2um is used Radiation: UV has limited penetration, so it is generally safe to use although it is less effective to X-rays and gamma rays. X-rays and gamma rays are used only for special purposes only Solvent: Solvent like ETHANOL and iso-propanol kills MICROBIAL cells but not the spores. The most common methods of sterilization practised in lab are: Dry heat: Specimen containing bacteria is exposed to high temperature Wet heat: Pressurised steam is used to kill microbes, for example, autoclave that is like pressure cooker that produces steam. Filtration: Filtration is used where filters are as small as 0.2um is used Radiation: UV has limited penetration, so it is generally safe to use although it is less effective to X-rays and gamma rays. X-rays and gamma rays are used only for special purposes only Solvent: Solvent like ethanol and iso-propanol kills microbial cells but not the spores. |
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