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What Do You Understand By Theoretical Plate Concept And How Hetp Affects The Separation Of Hplc Column?

Answer»

Plate theory concept was introduced to explain efficiency of columns. The concept ASSUMES that a state of instantaneous equilibrium EXISTS between the CONCENTRATION of solute in stationary phase and the mobile phase and further the column is imagined to be divided into a number of theoretical PLATES. Any analyte spends a finite time in each plate and this is the equilibrium time. Smaller the plate height the greater is the number of plates in a given LENGTH (HETP) and better is the column resolution.

Plate theory concept was introduced to explain efficiency of columns. The concept assumes that a state of instantaneous equilibrium exists between the concentration of solute in stationary phase and the mobile phase and further the column is imagined to be divided into a number of theoretical plates. Any analyte spends a finite time in each plate and this is the equilibrium time. Smaller the plate height the greater is the number of plates in a given length (HETP) and better is the column resolution.



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