What is freeze preservation ? Discuss any three types of freeze preser – InterviewSolution

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1.	What is freeze preservation ? Discuss any three types of freeze preservation.
Answer» Freeze Preservation: in this approach, cells and tissues are stored at - 196°C in liquid nitrogen. Theoretically, the cell and tissues can be stored in live state indefinitely, viability generally declines with the duration of cryopreservation, and thawed cells/tissues show variable degrees of damage, which they may be able to repair under appropriate media and culture conditions. It is desirable to preserve organised tissues like shoot-tips, and somatic and zygotic embryos to minimize the risk of genetic instability associated with unorganized tissues like cell suspensions and protoplasts.

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