When chromogenic substrate is added to the medium containing bacterial

1.	When chromogenic substrate is added to the medium containing bacterial colonies are subjected to insertional inactivation, some appears as blue coloured and others as white coloured colonies. Give reasons.
Answer» The colonies appears as blue coloured does not possess for eigengene they are called non-recombinants while the colonies of white coloured have foreign gene insert, they are called as recombinants. The production of blue colour is due to the reaction between beta-galactosidase enzyme and chromogenic substrate while the white coloured colonies have no enzyme for reaction with substrate.

When chromogenic substrate is added to the medium containing bacterial colonies are subjected to insertional inactivation, some appears as blue coloured and others as white coloured colonies. Give reasons.

Answer»

The colonies appears as blue coloured does not possess for eigengene they are called non-recombinants while the colonies of white coloured have foreign gene insert, they are called as recombinants.

The production of blue colour is due to the reaction between beta-galactosidase enzyme and chromogenic substrate while the white coloured colonies have no enzyme for reaction with substrate.

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When chromogenic substrate is added to the medium containing bacterial colonies are subjected to insertional inactivation, some appears as blue coloured and others as white coloured colonies. Give reasons.

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