Why gram negative do not take gram stain while gram positive takes it?

1.	Why gram negative do not take gram stain while gram positive takes it?
Answer» The\xa0Gram stain\xa0involves\xa0staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain.\xa0Both\xa0gram-positive and\xa0gram-negative cells have peptidoglycan in their cell walls, so initially,\xa0all bacteria stain\xa0violet.\xa0Gram staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safanin. Due to differences in the thickness of a peptidoglycan layer in the cell membrane between Gram positive and Gram negative bacteria, Gram positive bacteria (with a thicker peptidoglycan layer) retain crystal violet stain during the decolorization process, while Gram negative bacteria lose the crystal violet stain and are instead stained by the safranin in the final staining process.\xa0

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