Why is the 'insertional inactivation' method to detect recombinant DNA

1.	Why is the 'insertional inactivation' method to detect recombinant DNA preferred to 'antibiotic resistance' procedure ?
Answer» The presence of a chromogenic substrate gives blue coloured colonies, in absence of an insert/in non-transformants, presence of an insert (in the enzyme site), results into (insertional inactivation of the α-galactosidase) colonies which do not produce colour. Antibiotic resistance method requires duplicate plating/cumbersome procedure.

Why is the 'insertional inactivation' method to detect recombinant DNA preferred to 'antibiotic resistance' procedure ?

Answer»

The presence of a chromogenic substrate gives blue coloured colonies, in absence of an insert/in non-transformants, presence of an insert (in the enzyme site), results into (insertional inactivation of the α-galactosidase) colonies which do not produce colour.

Antibiotic resistance method requires duplicate plating/cumbersome procedure.

Discussion

No Comment Found

Related InterviewSolutions

In rDNA technology the alein DNA and cloning vector are a cut by same Restriction endo nuclease enzyme. Give reason.
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is calledA. ElectrophoresisB. ResolutionC. ElutionD. Spooling
A typical agarose gel electrophoresis showing migration of undigested (lane 1) and digested set DNA fragments (lane 2 to 4) is show below. Identify A, B ,C and D by selecting the option : A. A-DNA bnads , B-Wells ,C-Largest , D-SmallestB. A-Wells , B-DNA bands , C-Smallest, D-LargestC. A-Wells , B-DNA bands , C-Largest, D-SmallestD. A-Smallest, B-Wells , C-DNA bands, D-Largest
Agarose extracted from sea weeds finds use inA. Gel electrophoresisB. SpectrophotometryC. Tissue cultureD. PCR
Agarose extracted from sea weeds finds use inA. PCRB. Gel electrophoresisC. SpectrophotometryD. Tissue culture
A mixture containing DNA fragments a, b, c and d with molecular weigths of `a+b=c,a gt b` and `d gt c`, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would beA. B,A,C,DB. A,B,C,DC. C,B,A,DD. B,A,D,C
Which one is used as vector in genetic engineering ?A. PlasmidB. BacteriumC. CyanophageD. None of these
Agarose extracted from sea weeds finds use inA. Tissue cultureB. PCRC. Gel electrophoresisD. Spectrophotomery
Read the following four statements (A-D) A. The first transgenic buffalo, Roise produced milk which was human alpha-lactalbumin enriched B. Restriction enzymes are used in isolation of DNA from other macromolecules C. Downstream processing is one of the step of rDNA technology D. Disarmed pathogen vectors are also used in transfer of rDNA into the host which of the two statements have mistakes ?A. Statements (A) and (B)B. Statements(B) and (C)C. Statements (C) and (D)D. Statements (A) and (C)
Which one of the following techniques made it possible to gnetically engineer living organisms ?A. HybridizationB. Recombinant DNA techniquesC. X-ray diffractionD. Heavier isotope labelling

Why is the 'insertional inactivation' method to detect recombinant DNA preferred to 'antibiotic resistance' procedure ?

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment