1.

(1) State the type of technique shown in the diagram and its importance.(2) Identify the step occurring in the middle and its utility.(3) Mention the role of taq polymerase in the technique

Answer»
  1. The type of technique shown above is Polymerase Chain Reaction (PCR). It is used in.
    Recombinant DNA Technology (RDT) and in various other fields to amplify a small amount of DNA.
  2. The middle step occurring in the PCR is annealing where two small oligonucleotide primers are used to anneal or hybridise the complementary DNA strands.
  3. Taq polymerase is the Thermostable enzyme which has an ability to tolerate the high- temperature in the reaction to induce denaturation of double stranded DNA.


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