(a) A recombinant vector with a gene of interest inserted within the g

1.	(a) A recombinant vector with a gene of interest inserted within the gene of α-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.(b) Why is this method of selection referred to as "insertional inactivation" ?
Answer» (a) Bacteria I are grown in a medium with chrornogenic substrate,colonies formed show blue colour-no recombinants, no blue colour presence of recombinants. (b) Gene for the enzyme is inactivated by insertion.

(a) A recombinant vector with a gene of interest inserted within the gene of α-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.(b) Why is this method of selection referred to as "insertional inactivation" ?

Answer»

(a) Bacteria I are grown in a medium with chrornogenic substrate,colonies formed show blue colour-no recombinants, no blue colour presence of recombinants.

(b) Gene for the enzyme is inactivated by insertion.

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