a. Explain how to find whether an E. coli bacterium has transformed or

1.	a. Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it.b. What does the ampicillin resistant gene act as in the above case?
Answer» a. E. coli bearing transferred recombinant DNA are first grown on ampicillin containing medium and then transferred on to a medium containing tetracycline. The transformants will grow only in ampicillin containing medium and not in tetracycline containing medium. The non-transformants, on the other hand, will grow in both the mediums. b. Ampicillin resistant gene acts as a selectable marker and helps in selecting the transformants.

a. Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it.b. What does the ampicillin resistant gene act as in the above case?

Answer»

a. E. coli bearing transferred recombinant DNA are first grown on ampicillin containing medium and then transferred on to a medium containing tetracycline. The transformants will grow only in ampicillin containing medium and not in tetracycline containing medium. The non-transformants, on the other hand, will grow in both the mediums.

b. Ampicillin resistant gene acts as a selectable marker and helps in selecting the transformants.

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a. Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it.b. What does the ampicillin resistant gene act as in the above case?

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