InterviewSolution
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InterviewSolution
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a. Explain the significance of ‘palindromic nucleotide sequence’ in the formation of recombinant DNA.b. Write the use of restriction endonuclease in the above process. |
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Answer» a. Palindromic nucleotide sequence is the recognition (specific) sequence present both on the vector and on a desired or alien DNA for the action of the same (specific) restriction endonuclease to act upon. b. i. Every endonuclease inspects the entire DNA sequence for the palindromic recognition sequence. ii. On finding the palindrome, the endonuclease binds to the DNA. iii. It cuts the opposite strands of DNA in the sugar–phosphate backbone; a little away from the centre of the palindrome sites but between the same bases on both strands. iv. This results in the formation of single stranded overhanging stretches at the end of each strand called sticky ends. v. The sticky ends facilitate the action of the enzyme DNA ligase by readily forming hydrogen bonds with complementary strands. |
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