(a) Identify the selectable markers in the diagram of E. coli vector s

1.	(a) Identify the selectable markers in the diagram of E. coli vector shown above.(b) How is the coding sequence of a-galactosidase considered a better marker than the ones identified by you in the diagram? Explain.
Answer» (a) a–Ampicillin resistance d–Tetracycline. (b) The insertion of rDNA into the coding sequence of an enzyme a-galactosidase leads to the inactivation of the enzyme called insertional inactivation. The recombinants do not produce a blue coloured colonies in the presence of chromogenic substrate while the non-recombinants produce a blue colour. Thus, coding sequence of a-galactosidase is a better marker.

(a) Identify the selectable markers in the diagram of E. coli vector shown above.(b) How is the coding sequence of a-galactosidase considered a better marker than the ones identified by you in the diagram? Explain.

Answer»

(a) a–Ampicillin resistance

d–Tetracycline.

(b) The insertion of rDNA into the coding sequence of an enzyme a-galactosidase leads to the inactivation of the enzyme called insertional inactivation. The recombinants do not produce a blue coloured colonies in the presence of chromogenic substrate while the non-recombinants produce a blue colour. Thus, coding sequence of a-galactosidase is a better marker.

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(a) Identify the selectable markers in the diagram of E. coli vector shown above.(b) How is the coding sequence of a-galactosidase considered a better marker than the ones identified by you in the diagram? Explain.

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