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1. |
A protein from cell A and cell B is compared to find the whole protein pattern by a technique developed by O’Farrel. Name the technique. State its principle. |
Answer» Two dimensional gel electrophoresis. The proteins are separated by their isoelectric points (pI) in the first dimension (IEF), and according to their molecular mass in the second dimension (SDS-PAGE). Cumulative effects of these techniques lead to high resolution |
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