1.

A protein from cell A and cell B is compared to find the whole protein pattern by a technique developed by O’Farrel. Name the technique. State its principle. 

Answer»

Two dimensional gel electrophoresis.

The proteins are separated by their isoelectric points (pI) in the first dimension (IEF), and according to their molecular mass in the second dimension (SDS-PAGE).

Cumulative effects of these techniques lead to high resolution 



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