A selectable marker is used in the section of recombinants on the basi

1.	A selectable marker is used in the section of recombinants on the basis of their ability to produce colour in presence of chromogenic substrate. (a) Mention the name of mechanism involved. (b) Which enzyme is involved in production of colour? (c) How is it advantageous over using antibiotic resistant gene as a selectable marker?
Answer» (a) Insertional inactivation (b) galactosidase. (c) Selection of recombinants due to inactivation of antibiotics requires simultaneous plating on two plates having different antibiotics.

A selectable marker is used in the section of recombinants on the basis of their ability to produce colour in presence of chromogenic substrate. (a) Mention the name of mechanism involved. (b) Which enzyme is involved in production of colour? (c) How is it advantageous over using antibiotic resistant gene as a selectable marker?

Answer»

(a) Insertional inactivation

(b) galactosidase.

(c) Selection of recombinants due to inactivation of antibiotics requires simultaneous plating on two plates having different antibiotics.

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