A specific DNA sequence is identified from a heterogeneous population

1.	A specific DNA sequence is identified from a heterogeneous population of DNA molecules on the basis of DNA-DNA hybridization. (a) Identify the technique. (b) Schematically depict the steps of DNA separation technique.
Answer» (a) Southern hybridization (b) (i) Digestion of genomic DNA with restriction enzymes (ii) Electrophoresis on agarose gel (iii) Transfer to a nitrocellulose membrane (Southern Blotting) (iv) Hybridization with labeled probe (v) Detection of labeled probe.

A specific DNA sequence is identified from a heterogeneous population of DNA molecules on the basis of DNA-DNA hybridization. (a) Identify the technique. (b) Schematically depict the steps of DNA separation technique.

Answer»

(a) Southern hybridization

(b) (i) Digestion of genomic DNA with restriction enzymes

(ii) Electrophoresis on agarose gel

(iii) Transfer to a nitrocellulose membrane (Southern Blotting)

(iv) Hybridization with labeled probe

(v) Detection of labeled probe.

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A specific DNA sequence is identified from a heterogeneous population of DNA molecules on the basis of DNA-DNA hybridization. (a) Identify the technique. (b) Schematically depict the steps of DNA separation technique.

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