(a) Write the palindromic nucleotide sequence for the following DNA se

1.	(a) Write the palindromic nucleotide sequence for the following DNA segment:5′ - GAATTC - 3′(b) Name the restriction endonuclease that recognizes this sequence. (c) How are ‘sticky ends’ produced? Mention their role
Answer» (a) 5' – GAATTC – 3' 3' – CTTAAG – 5' (b) EcoRI is the restriction endonuclease that recognizes this palindrome. (c) When the restriction enzyme cuts the DNA strands a little away from the center of the palindromic sequence, between the same two bases on both the strands, sticky ends are produced. The stickiness of the ends facilitates the action of the enzyme DNA-ligase.

(a) Write the palindromic nucleotide sequence for the following DNA segment:5′ - GAATTC - 3′(b) Name the restriction endonuclease that recognizes this sequence. (c) How are ‘sticky ends’ produced? Mention their role

Answer»

(a) 5' – GAATTC – 3'

3' – CTTAAG – 5'

(b) EcoRI is the restriction endonuclease that recognizes this palindrome.

(c) When the restriction enzyme cuts the DNA strands a little away from the center of the palindromic sequence, between the same two bases on both the strands, sticky ends are produced.

The stickiness of the ends facilitates the action of the enzyme DNA-ligase.

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(a) Write the palindromic nucleotide sequence for the following DNA segment:5′ - GAATTC - 3′(b) Name the restriction endonuclease that recognizes this sequence. (c) How are ‘sticky ends’ produced? Mention their role

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