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Applications of buffer in analytical chemistry

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Buffer SOLUTIONS are used as a means of keeping pH at a nearly constant value in a wide VARIETY of chemical applications. In nature, there are MANY systems that use buffering for pH regulation. For example, the bicarbonate buffering SYSTEM is used to regulate the pH of blood.

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blood contains a carbonate/bicarbonate buffer that keeps the pH close to 7.4. Enzyme activity depends on pH, so the pH during an enzyme assay must stay constant

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