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Assertion : Recognition site should be perfectly single and reponsive to commonly used restriction enzymes. Reason: In pNR 322 Alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.A. If both the assertion and the reason are true and the reason is a correct explanation of the assertion.B. If both the assertion and reason are true but the reason is not a correct explanation of the assetionC. If the assertion is true but the reason is falseD. If the both the assertion and reason are false

Answer» Correct Answer - b
b) Recognition site or site where alien DNA can be inserted should be single because occurrence of more than one recognition site will cause DNA fragmentation and disturb gene cloning.


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