Assertion : Use of chitinase enzyme is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason : Fungal cell wall is made up of fungal cellulose or chitin.

Assertion : Use of chitinase enzyme is necessary for isolation of DNA

1.	Assertion : Use of chitinase enzyme is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason : Fungal cell wall is made up of fungal cellulose or chitin.
Answer» Correct Answer - A Release of DNA from a cell is achieved by treating the cells or tissues with enzymes such as lysozyme (bacterial), cellulose (plant cells), chitinase (fungus), etc. These enzymes degrade cell wall, plasma membrane degrading enzymes like lipase, etc. are also needed. Since yeast is a fungus and fungal cell with is made of chitin (fungal cellulose), isolation of DNA necessarily requires use of enzyme chitinase.

Discussion

No Comment Found

Related InterviewSolutions

In rDNA technology the alein DNA and cloning vector are a cut by same Restriction endo nuclease enzyme. Give reason.
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is calledA. ElectrophoresisB. ResolutionC. ElutionD. Spooling
A typical agarose gel electrophoresis showing migration of undigested (lane 1) and digested set DNA fragments (lane 2 to 4) is show below. Identify A, B ,C and D by selecting the option : A. A-DNA bnads , B-Wells ,C-Largest , D-SmallestB. A-Wells , B-DNA bands , C-Smallest, D-LargestC. A-Wells , B-DNA bands , C-Largest, D-SmallestD. A-Smallest, B-Wells , C-DNA bands, D-Largest
Agarose extracted from sea weeds finds use inA. Gel electrophoresisB. SpectrophotometryC. Tissue cultureD. PCR
Agarose extracted from sea weeds finds use inA. PCRB. Gel electrophoresisC. SpectrophotometryD. Tissue culture
A mixture containing DNA fragments a, b, c and d with molecular weigths of `a+b=c,a gt b` and `d gt c`, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would beA. B,A,C,DB. A,B,C,DC. C,B,A,DD. B,A,D,C
Which one is used as vector in genetic engineering ?A. PlasmidB. BacteriumC. CyanophageD. None of these
Agarose extracted from sea weeds finds use inA. Tissue cultureB. PCRC. Gel electrophoresisD. Spectrophotomery
Read the following four statements (A-D) A. The first transgenic buffalo, Roise produced milk which was human alpha-lactalbumin enriched B. Restriction enzymes are used in isolation of DNA from other macromolecules C. Downstream processing is one of the step of rDNA technology D. Disarmed pathogen vectors are also used in transfer of rDNA into the host which of the two statements have mistakes ?A. Statements (A) and (B)B. Statements(B) and (C)C. Statements (C) and (D)D. Statements (A) and (C)
Which one of the following techniques made it possible to gnetically engineer living organisms ?A. HybridizationB. Recombinant DNA techniquesC. X-ray diffractionD. Heavier isotope labelling

Assertion : Use of chitinase enzyme is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason : Fungal cell wall is made up of fungal cellulose or chitin.

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment