. Auto immunity5. Given below is the figure of a cloning vector pBR322 in which a foreign DNA isligated at the Pvu l site. Which antibiotic resistance gene helps in selection ofrecombinants?Pvu IPstBamH 1amptetpBR322orfPvu IItet geneampRgene. EcoRie rop

. Auto immunity5. Given below is the figure of a cloning vector pBR322

1.	. Auto immunity5. Given below is the figure of a cloning vector pBR322 in which a foreign DNA isligated at the Pvu l site. Which antibiotic resistance gene helps in selection ofrecombinants?Pvu IPstBamH 1amptetpBR322orfPvu IItet geneampRgene. EcoRie rop
Answer» Answer: ampR geneExplanation:The cloning sites are actually the specific unique recognition sequence for a particular restriction enzyme, so as to link the foreign DNA with the vector DNA to create a recombinant DNA molecules, (l) These sites are important for joining of DNA fragments of vector and alien DNA. And also multiple recognition sequences for a particular restriction enzyme within a DNA or vector will complicate the process of gene cloning.The two cloning sites in pBR322 are Bam HI of tetracycline resistant gene and Pvu I of ampicillin resistant genes