(Biology) 60Biology for Class-XIIQ26. What is Gel Electrophoresis? On

1.	(Biology) 60Biology for Class-XIIQ26. What is Gel Electrophoresis? On what principledoes the technique work ? Mention one way by which theproducts obtained through this technique can be utilised.
Answer» Agarose gel electrophoresisis a method ofgel electrophoresisused inbiochemistry,molecular biology,genetics, andclinical chemistryto separate a mixed population of macromolecules such as DNA or proteins in a matrix ofagarose, one of the two main components ofagar Mechanism of migration and separation. The negative charge of its phosphate backbone moves the DNA towards the positively charged anode during electrophoresis. However, the migration of DNA molecules insolution, in the absence of a gel matrix, is independent of molecular weight during electrophoresis Uses : a. The purified DNA fragments obtained by gel electrophoresis.b. They are used in DNA fingerprinting.

(Biology) 60Biology for Class-XIIQ26. What is Gel Electrophoresis? On what principledoes the technique work ? Mention one way by which theproducts obtained through this technique can be utilised.

Answer»

Agarose gel electrophoresisis a method ofgel electrophoresisused inbiochemistry,molecular biology,genetics, andclinical chemistryto separate a mixed population of macromolecules such as DNA or proteins in a matrix ofagarose, one of the two main components ofagar

Mechanism of migration and separation. The negative charge of its phosphate backbone moves the DNA towards the positively charged anode during electrophoresis. However, the migration of DNA molecules insolution, in the absence of a gel matrix, is independent of molecular weight during electrophoresis

Uses : a. The purified DNA fragments obtained by gel electrophoresis.b. They are used in DNA fingerprinting.

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