1.

Can DNA polymerase extend the chain which has 5'OH on the growing end ? if yes/no ...please explain ​

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Answer:

I understand that the polymerase MOVES from 5' to 3' (based on the orientation of the carbon molecule), but I don't understand why it would create the Okazaki fragments at all, then. If it can only attach things to the 3' end, why does it attach to the lagging strand? Wouldn't it not be able to move at all to create any kind of FRAGMENT in the lagging strand? And in the same vein, once it starts replication in that direction, if all of the CARBONS are going that same "wrong" direction in the lagging strand, why would the polymerase be forced to stop at a certain point to create the fragments? I understand that it reaches a new primer, but once it starts replicating then shouldn't it not have to stop?

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