Can I Get A Sharper Peak By Injecting My Sample In A Weaker Injection

1.	Can I Get A Sharper Peak By Injecting My Sample In A Weaker Injection Solvent?
Answer» In this scenario, the SAMPLE is initially concentrated onto the head of the column and moves through the column in a TIGHT band. This technique is sometimes USED to minimize band broadening for a larger volume sample injection. Keep in mind that your sample components must be soluble in the mobile PHASE as WELL, in order for this to work.

Can I Get A Sharper Peak By Injecting My Sample In A Weaker Injection Solvent?

Answer»

In this scenario, the SAMPLE is initially concentrated onto the head of the column and moves through the column in a TIGHT band.
This technique is sometimes USED to minimize band broadening for a larger volume sample injection.
Keep in mind that your sample components must be soluble in the mobile PHASE as WELL, in order for this to work.