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Can I Get A Sharper Peak By Injecting My Sample In A Weaker Injection Solvent (such As 100% Water For Reverse Phase)? |
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Answer» In this SCENARIO, the sample is initially concentrated onto the head of the column and moves through the column in a tight band. This technique, REFERRED to as “on-column compression” or “point-injected”, is SOMETIMES used to minimize band broadening for a larger VOLUME sample injection. Keep in MIND that your sample components must be soluble in mobile phase as well, in order for this to work. In this scenario, the sample is initially concentrated onto the head of the column and moves through the column in a tight band. This technique, referred to as “on-column compression” or “point-injected”, is sometimes used to minimize band broadening for a larger volume sample injection. Keep in mind that your sample components must be soluble in mobile phase as well, in order for this to work. |
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