Define :(a) Complementary DNA (cDNA) (b) Complementary sequence(c)

1.	Define :(a) Complementary DNA (cDNA) (b) Complementary sequence(c) Cosmid
Answer» (a) Complementary DNA (cDNA) DNA that is synthesized in the laboratory from a messenger RNA template. (b) Complementary sequence Nucleic acid base sequence that can form a double-stranded structure with another DNA fragment by following base-pairing rules (A pairs with T and C with G). The complementary sequence to GTAC for example, is CATG. (c) Cosmid Artificially constructed cloning vector containing the cos gene of phage lambda. Cosmids can be packaged in lambda phage particles for infection into E. coli; Permits cloning of larger DNA fragments (up to 45 kb) than can be introduced into bacterial hosts in plasmid vectors.

Define :(a) Complementary DNA (cDNA) (b) Complementary sequence(c) Cosmid

Answer»

(a) Complementary DNA (cDNA)

DNA that is synthesized in the laboratory from a messenger RNA template.

(b) Complementary sequence

Nucleic acid base sequence that can form a double-stranded structure with another DNA fragment by following base-pairing rules (A pairs with T and C with G). The complementary sequence to GTAC for example, is CATG.

(c) Cosmid

Artificially constructed cloning vector containing the cos gene of phage lambda. Cosmids can be packaged in lambda phage particles for infection into E. coli; Permits cloning of larger DNA fragments (up to 45 kb) than can be introduced into bacterial hosts in plasmid vectors.

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