1.

Define totipotency of a cell. List the requirements if the objective is to produce somaclones of a tomato plant on commercial scale.

Answer»

The capacity of a cell/explant to grow into a whole plant is called totipotency.

Requirements:

i. Explant: Any part of a plant taken out and grown in a test tube.

ii. Nutrient medium: It must have carbon source (sucrose), inorganic salts, growth regulators (auxins, cytokinins, etc.), vitamins and amino acids.

iii. Suitable light and temperature conditions.



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