Describe the important characteristics a cloning vector must possess.

1.	Describe the important characteristics a cloning vector must possess.
Answer» To force a recombinant DNA into host cells, a cloning vector must possess the following characteristics: (a) (Origin of replication (ori): The process of replication begins from here where any piece of DNA when linked to this sequence is made to replicate within the host cells. (b) Selectable marker: It selectively permits only the growth of the transformants by identifying or selecting the transformants and eliminating non-transformants. (c) Cloning sites: These are the sites which a vector requires to link a foreign or alien DNA for commonly used restriction enzyme, e.g., restriction sites in E. Coli cloning vector includes Hindi II, Ecor I, BamW II, Sal I, Prul etc.

Answer»

To force a recombinant DNA into host cells, a cloning vector must possess the following characteristics:
(a) (Origin of replication (ori): The process of replication begins from here where any piece of DNA when linked to this sequence is made to replicate within the host cells.

(b) Selectable marker: It selectively permits only the growth of the transformants by identifying or selecting the transformants and eliminating non-transformants.

(c) Cloning sites: These are the sites which a vector requires to link a foreign or alien DNA for commonly used restriction enzyme, e.g., restriction sites in E. Coli cloning vector includes Hindi II, Ecor I, BamW II, Sal I, Prul etc.

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