An enzyme like any protein has primary structure (amino acid sequence). Secondary structure and tertiary structure
Every enzyme has an ’active site’ which is a crevice into which the substrate fits.
Enzymes are specific in their action. Particular enzyme acts on a particular substrate. But different enzymes catalyse a unique chemical or metabolic reaction.
Enzymes generally function in a narrow range of temperature and pH. Activity declines both below and above optimum temperature and pH.
The higher the affinity of the enzyme for its substrate the greater is its catalytic activity.
The activity of an enzyme is also sensitive to the presence of specific chemicals that bind to the enzyme.
For eg: Inhibitors that shuts off enzyme activity and Co-factors that facilitates catalytic activity.
Enzymes retain their identity at the end of the reaction