Describe the principle of MALDI technique. Write its two main uses in

1.	Describe the principle of MALDI technique. Write its two main uses in protein studies.
Answer» A popular method called Matrix Assisted Laser Desorption Ionisation (MALDI) is used to volatalise and protonate peptides and proteins. In this procedure, the sample is transferred from a condensed phase to a gas phase with the help of a solid matrix. This technique determines the molecular weight of proteins by separating molecular ions according to their mass/charge ratio. Uses: To obtain protein structural information such as peptide mass or amino acid sequences. To identify the type and location of amino acid modification within proteins.

Describe the principle of MALDI technique. Write its two main uses in protein studies.

Answer»

A popular method called Matrix Assisted Laser Desorption Ionisation (MALDI) is used to volatalise and protonate peptides and proteins. In this procedure, the sample is transferred from a condensed phase to a gas phase with the help of a solid matrix. This technique determines the molecular weight of proteins by separating molecular ions according to their mass/charge ratio.

Uses: To obtain protein structural information such as peptide mass or amino acid sequences.

To identify the type and location of amino acid modification within proteins.

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