Describe the roles of heat, primers and the bacterium Thermus aquaticu

1.	Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR.
Answer» Role of Heat : It helps in denaturation process in PCR. The ds DNA are heated in this process at very high temperature (95°C) so that both the strands separate. Role of primers : Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of new chain. Role of Bacterium Thermus aquaticus : From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms new strand.

Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR.

Answer»

Role of Heat : It helps in denaturation process in PCR. The ds DNA are heated in this process at very high temperature (95°C) so that both the strands separate.

Role of primers : Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of new chain.

Role of Bacterium Thermus aquaticus : From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms new strand.

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