Describe the steps in producing somatic hybrids from protoplast.

1.	Describe the steps in producing somatic hybrids from protoplast.
Answer» It is carried out by the following steps: i. Single cells from selected plants are isolated. ii. The cell walls of cells are digested by enzymes like pectinase and cellulase, to expose the naked protoplasts. iii. Naked protoplasts surrounded only by plasma membranes are isolated. iv. The isolated protoplasts are fused to obtain hybrid protoplasts under sterile conditions in special nutrient media. v. The hybrid protoplasts are cultured in a suitable media to form new plant.

Answer»

It is carried out by the following steps:

i. Single cells from selected plants are isolated.

ii. The cell walls of cells are digested by enzymes like pectinase and cellulase, to expose the naked protoplasts.

iii. Naked protoplasts surrounded only by plasma membranes are isolated.

iv. The isolated protoplasts are fused to obtain hybrid protoplasts under sterile conditions in special nutrient media.

v. The hybrid protoplasts are cultured in a suitable media to form new plant.

Discussion