Different methods have been suggested to introduce alien DNA into host

1.	Different methods have been suggested to introduce alien DNA into host cells. Given and explain any three methods adopted for this purpose.
Answer» (1) Micro-injection-recombinant DNA is directly injected into the nucleus of an animal cell. (2) Biolistics or gene gun – cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA. It is suitable for plants. (3) Disarmed pathogen – vectors when allowed to infect the cell, transfer the recombinant DNA into the host.

Different methods have been suggested to introduce alien DNA into host cells. Given and explain any three methods adopted for this purpose.

Answer»

(1) Micro-injection-recombinant DNA is directly injected into the nucleus of an animal cell.

(2) Biolistics or gene gun – cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA. It is suitable for plants.

(3) Disarmed pathogen – vectors when allowed to infect the cell, transfer the recombinant DNA into the host.

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Different methods have been suggested to introduce alien DNA into host cells. Given and explain any three methods adopted for this purpose.

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