Differentiate between: (i) PCR and Gene Cloning. (ii) Batch Culture

1.	Differentiate between: (i) PCR and Gene Cloning. (ii) Batch Culture and Continuous Culture.
Answer» (i) PCR: • Amplification of desired segment of DNA within hours. • No host cell required. Gene cloning: • Amplification of a desirable gene takes more time under lab condition. • Host cell is required. (ii) Batch culture: • In this culture, the same medium and all the cells produced are retained in the culture vessel. Fresh medium is not added. • The cell number of biomass exhibits a typical sigmoid curve. Continuous culture: • In this culture, both cells and used medium are taken out from the continuous culture and replaced by equal volume of fresh medium. • Here, the cell population is a maintained in a steady state by regularly replacing a portion of used or spent medium.

Differentiate between: (i) PCR and Gene Cloning. (ii) Batch Culture and Continuous Culture.

Answer»

(i) PCR:

• Amplification of desired segment of DNA within hours.

• No host cell required.

Gene cloning:

• Amplification of a desirable gene takes more time under lab condition.

• Host cell is required.

(ii) Batch culture:

• In this culture, the same medium and all the cells produced are retained in the culture vessel. Fresh medium is not added.

• The cell number of biomass exhibits a typical sigmoid curve.

Continuous culture:

• In this culture, both cells and used medium are taken out from the continuous culture and replaced by equal volume of fresh medium.

• Here, the cell population is a maintained in a steady state by regularly replacing a portion of used or spent medium.