

InterviewSolution
1. |
Distinguish between:(i) dNTP & ddNTP(ii) pBR322 & pUC19(iii) M-13 & lambda phage(iv) Cosmid & plasmid(v) Transformation and transfection |
Answer» (i) dNTP & ddNTP dNTP used in DNA replication ddNTP used in chain termination Structural differences can also be shown (ii) pBR322 & pUC19 pBR322 - contains two antibiotic resistant genes pUC19 - contains MCS (iii) M-13 & lambda phage M-13 : Circular and single stranded Lambda phage : Linear double stranded (iv) Cosmid & plasmid Cosmid : having features of plasmid and cos sites of phage lambda Plasmid : small, circular, extra-chromosomal self replicating naturally present in bacteria (v) Transformation and transfection Transformation: Cold CaCl2 treated competent bacterial cells to introduce rDNA Transfection: Transfer rDNA into host cells by mixing foreign DNA with charged substances like calcium liposomes/calcium phosphate/DEAE dextran |
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