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During ultracentrifugation of cell homogenate, which fraction would be separated at `10000gxx20` minutes?A. Mitochondria and lysosomesB. ribosomes and microsomeC. nucleus and mitochondriaD. endoplasmic reticulum |
Answer» Correct Answer - A At 400-500`xxg` speed it yields a pellet consisting of unbroken tissue, whole cells, cell nuclei and large debris. 10000-20000`xxg` brings mitochondria, lyososomes and peroxisomes. 80000`xxg` yields microsomal pellet, while at 150000-300000`xxg` ribosomes and macromolecules sediment. |
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