ELISA technique is based on the principles of antigen and antibody int – InterviewSolution

1.	ELISA technique is based on the principles of antigen and antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
Answer» Yes. One can use antibody against the enzyme (that is responsible for the metabolism of phenylalanine) to develop ELISA-based diagnostic technique. The patient in which the enzyme protein is absent would give negative result in ELISA when compared to normal individual.

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