InterviewSolution
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InterviewSolution
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Enlist two names of restriction endonucleases with their restriction sites |
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Answer» The orthodox TYPE II restriction endonuclease is a homodimer of ∼2 × 30 kDa molecular MASS, which recognizes a palindromic sequence 4–8 bp in length, and in the presence of Mg2+ cleaves the TWO strands of the DNA WITHIN or immediately adjacent to the recognition site to give a 5′-phosphate and a 3′-OH END. |
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