1.

Enumerate the assumptions that we undertaker in making the respiratory balance Sheet . Are these assumptions valid for a living system? Compare fermentation and aerobic respiration in this context.

Answer» The Calculations of the net gain of ATP for every glucose molecules oxidised can be made on the following assumptions
(i) There is sequential pathway that follows. i.e., glycolysis , TCA cycle and ETS in cytoplasm , mitochndrial matrix and inner mitochonodrial membrane respectively.
(ii) NADH, synthesised in glycolysis enters in to ETC for phosphorylation.
(iii) None of the intermediates in the pathway are utlised to synthesis any other compound
(iv) Glucose forms respiatory substrate.
These assumptions are not valid for a living system because of following neasons
(i) These all pathways work simultaneously and do not take place one after the other.
(ii) ATP is utilised when needed.
(iii) Rate of enzyme actions is controlled by multiple means.
Comparisan between fermentation and aerobic respiration are as follows
`{:("Fermentation","Aerobic Respirartion"),("Fermentation is partial breakdown of glucose.","It is complete breakdown of glucose"),("Net gain of only "2ATP,38 ATP " are produced"),("Oxidation of NADH to " NAD^(+) " is slow proces","It is vigorous reaction in aerobic respiration"):}`


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