Saved Bookmarks
| 1. |
Enumerate the major steps of isolation of DNA. |
|
Answer» Major steps for isolation of DNA 1. Cell containing DNA is treated with lysozyme or cellulose or chitinase 2. DNA along with RNA, Protein, lipid are released 3. Treatment with RNAase, protease to remove RNA and Protein 4. Appropriate treatment to remove other impurities 5. Addition of chilled ethanol to get precipitation of purified DNA 6. The fine threads of DNA can be collected by centrifugation |
|