Explain about the principle involved in chromatography. Give its types

1.	Explain about the principle involved in chromatography. Give its types.
Answer» Solution :(i) The principle behind chromatography is selective distribution of the MIXTURE of organic substances between TWO phases-a stationary phase and a moving phase. The stationary phase can be a solid or liquid while the moving phase is a liquid or a gas. (II) If the stationary phase is solid, the basis is adsorption and when it is a liquid, the basis is partition. (III) Chromatography is defined as technique for the separation of a mixture brought about by differential movement of the individual compound through porous medium under the influence of moving solvent. (iv) The various methods of chromatography are: 1. Column chromatography (CC) 2. Thin layer chromatography (TLC) 3. Paper chromatography (PC) 4. Gas liquid chromatography (GLC) 5. Ion exchange chromatography

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